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Sample GSM405316 Query DataSets for GSM405316
Status Public on Apr 26, 2010
Title glioma 243
Sample type RNA
 
Source name brain tissue, glioma
Organism Homo sapiens
Characteristics tissue: brain
gender: Male
histology: OA (grade III)
age at diagnosis: 40.06
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description Gene expression analysis.
Data processing Expression levels of 17,527 genes were extracted from Affymetrix HU133 Plus 2.0 arrays using updated array annotation (Dai et al., 2005) based on reorganizing probes to gene specific probe sets, excluding inaccurate or wrongly annotated probes.
 
Submission date May 19, 2009
Last update date Apr 26, 2010
Contact name Pim French
E-mail(s) p.french@erasmusmc.nl
Phone +31 10 70443 33
Organization name Erasmus MC
Department Neurology
Lab Neurooncology
Street address Dr Molewaterplein 50
City Rotterdam
ZIP/Postal code 2040CA
Country Netherlands
 
Platform ID GPL8542
Series (1)
GSE16011 Intrinsic Gene Expression Profiles of Gliomas are a Better Predictor of Survival than Histology

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity

Data table
ID_REF VALUE
10000_at 5.977052627
10001_at 8.236255077
10002_at 4.917560331
10003_at 4.503890286
10004_at 5.519609294
10005_at 7.936614807
10006_at 9.513980652
10007_at 9.132415492
10008_at 6.307182359
10009_at 8.60507419
1000_at 9.828224228
10010_at 8.754264678
10011_at 8.3596921
10013_at 7.588140482
10014_at 7.459692223
10015_at 9.12477331
10016_at 10.00012051
10017_at 5.400152892
10018_at 5.34349806
10019_at 8.568589386

Total number of rows: 17527

Table truncated, full table size 354 Kbytes.




Supplementary file Size Download File type/resource
GSM405316.CEL.gz 5.0 Mb (ftp)(http) CEL
GSM405316.chp.gz 375.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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