|
| Status |
Public on Aug 06, 2019 |
| Title |
3_Mock_DEM |
| Sample type |
SRA |
| |
|
| Source name |
MEFs_mock_DEM
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibroblast
genotype/variation: MafF-/-::MafG-/-::MafK-/-
treatment: 100µM Diethyl Maleate (DEM) for 12 hrs
|
| Treatment protocol |
MEFs were treated with 100µM Diethyl Maleate (DEM) or Vehcle for 12 hr.
|
| Growth protocol |
MEFs were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared by using RNeasy Mini Kit (QIAGEN).
Isolation of poly(A)-tailed RNA and library construction were performed using SureSelect Strand Specific RNA Sample Prep Kit (Agilent Technologies).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
000545Y
|
| Data processing |
Illumina bcl2fastq v2.16.0.10 was used for basecalling.
Reads were mapped against mouse (mm9) genome using the TopHat v2.0.8b.
Differential gene analaysis was performed using Cuffdiff v2.1.1.
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each genotype were generated using Cuffdiff.
|
| |
|
| Submission date |
Jul 16, 2019 |
| Last update date |
Aug 06, 2019 |
| Contact name |
Fumiki Katsuoka |
| E-mail(s) |
kfumiki@med.tohoku.ac.jp
|
| Organization name |
Tohoku University
|
| Street address |
2-1 Seiryo-cho
|
| City |
Aoba-ku, Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8573 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE134351 |
Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq] |
| GSE134353 |
Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells |
|
| Relations |
| BioSample |
SAMN12284759 |
| SRA |
SRX6451601 |
