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| Status |
Public on Aug 06, 2019 |
| Title |
Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To elucidate the function of Nrf2-small Maf heterodimer, we constructed a tethered Nrf2-MafG (T-N2G) heterodimer using a flexible linker peptide and examined its function in the small Maf-deficient mouse embryonic fibroblast (MEF).
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| Overall design |
polyA RNA-seq was performed using RNA from Immortalized MafF-/-::MafG-/-::MafK-/- (F0G0K0) MEFs and T-N2G-expressing F0G0K0 MEFs, treated with 100µM Diethyl Maleate (DEM) or Vehcle for 12hr.
sample title - condition - replicate (in the genes.read_group_tracking.txt)
2_Mock_Veh - Mock_Veh - 1
1_Mock_Veh - Mock_Veh - 0
3_Mock_Veh - Mock_Veh - 2
2_Mock_DEM - Mock_DEM - 1
1_Mock_DEM - Mock_DEM - 0
3_Mock_DEM - Mock_DEM - 2
3_T-N2G_Veh - N2_Veh - 2
1_T-N2G_Veh - N2_Veh - 0
2_T-N2G_Veh - N2_Veh - 1
1_T-N2G_DEM - N2_DEM - 0
2_T-N2G_DEM - N2_DEM - 1
3_T-N2G_DEM - N2_DEM - 2
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| Contributor(s) |
Katsuoka F, Otsuki A, Takahashi M, Ito S, Yamamoto M |
| Citation(s) |
31383749 |
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| Submission date |
Jul 16, 2019 |
| Last update date |
Nov 12, 2021 |
| Contact name |
Fumiki Katsuoka |
| E-mail(s) |
kfumiki@med.tohoku.ac.jp
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| Organization name |
Tohoku University
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| Street address |
2-1 Seiryo-cho
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| City |
Aoba-ku, Sendai |
| State/province |
Miyagi |
| ZIP/Postal code |
980-8573 |
| Country |
Japan |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE134353 |
Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells |
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| Relations |
| BioProject |
PRJNA554840 |
| SRA |
SRP214807 |
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