|
| Status |
Public on Oct 07, 2019 |
| Title |
Non inflamed mucosa from UC patient 7 |
| Sample type |
RNA |
| |
|
| Source name |
Intestinal mucosal biopsies
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Ulcerative Colitis
tissue: Intestinal mucosal biopsies
inflammation: Non Inflamed
mayo score: 3
|
| Treatment protocol |
Human biopsies taken from colonoscopy procedures were placed in RNA Later solution and then stored in -80ºC freezer until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA for microarrays was obtained using Mirvana microRNA Kit (Life Technologies Carlsbad, CA) according to manufacturer instructions, and RNA integrity was evaluated with the High Sensitivity RNA Analysis Kit (Advanced Analytical Technologies Ankeny, IA).
|
| Label |
biotin
|
| Label protocol |
Starting with 350 ng of total RNA, the Affymetrix FlashTagTMBiotin HSR labeling was used to label RNA according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
On average thirteen micrograms of labeled sample were hybridized for 16 hours at 48°C using the Affymetrix Eukaryotic Target Hybridization Controls protocol. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash, and Stain Kit was used according the manual.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
miRNA expression data from total intestinal mucosal biopsy
Colon RNA from Glucocorticoid non-inflamed biopsy (Gni) 3
|
| Data processing |
Data was obtained using the GeneChip® Command Console Software (AGCC; Version 3.2).
|
| |
|
| Submission date |
Jun 20, 2019 |
| Last update date |
Oct 07, 2019 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL19117 |
| Series (2) |
| GSE133059 |
miRNA expression data from a cohort of Chilean Ulcerative Colitis human intestinal samples |
| GSE133061 |
Gene and miRNA expression data from a cohort of Chilean Ulcerative Colitis human intestinal samples |
|
