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Sample GSM3815056 Query DataSets for GSM3815056
Status Public on Jan 31, 2020
Title BS27_cDC2
Sample type SRA
Source name kidney
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: kidney
age: 10 weeks
genotype: Clec9a-Cre/Cre Rosa-YFP/YFP
cell type: cDC2
sorting strategy: CD45+ Ly6C- MHCII+ CD11c+ CD64- CD11b+ YFP+
Extracted molecule polyA RNA
Extraction protocol RNA isolation with PicoPure Kit according to protocol. Cells were sorted directly in lysis buffer supplied with the kit
cDNA creation with SMARTseq v4 Kit (Takara), library creation with MicroPlex Library Preparation Kit v2 (Diagenode)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Data processing Sequencing reads were aligned to mm10 mouse genome using STAR
Gene expression in transcripts per kilobase million (TPM) was calculated with RSEM
Genome_build: mm10
Supplementary_files_format_and_content: comma separated value file contains TPM values for each sample, *tab files contain read counts.
Submission date May 24, 2019
Last update date Jun 10, 2020
Contact name Barbara Schraml
Organization name LMU Munich
Street address GroƟhaderner Strasse 9
City Planegg
ZIP/Postal code 82152
Country Germany
Platform ID GPL18480
Series (1)
GSE131751 RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice
BioSample SAMN11845944
SRA SRX5892967

Supplementary file Size Download File type/resource 172.0 Kb (ftp)(http) TAB
GSM3815056_BS27_SR18_042_cDC2_YFPpos_SR18_036_rsem.genes.results.txt.gz 441.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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