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Series GSE131751 Query DataSets for GSE131751
Status Public on Jan 31, 2020
Title RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We use bulk RNA sequencing of sorted cells to characterize the gene expression profiles of renal dendritic cell (DC) subsets, cDC1 and cDC2, as well as MHCII+CD64+ F4/80hi and MHCII+CD64+ CD11bhi cells. Splenic DCs and red pulp macrophages serve as reference populations for a macrophage-like or DC-like phenotype. By sorting YFP+/YFP- F4/80hi or YFP+/YFP- CD11bhi cells from Clec9a-Cre Rosa-YFP mice we aim to reveal transcriptional differences between YFP-labelled and unlabelled cells. We showed that F4/80hi cells resemble macrophages on a transcriptional level, despite their DC origin, and that renal CD11bhi cells are a transcriptionally unique subset. However, we were not able to reveal differences between YFP+ and YFP- populations.
 
Overall design RNAsequencing of murine dendritic cell and macrophage populations from kidney and spleen
 
Contributor(s) Rambichler S, Salei N, Schraml BU
Citation(s) 31932472
Submission date May 24, 2019
Last update date Jun 10, 2020
Contact name Barbara Schraml
E-mail(s) barbara.schraml@med.uni-muenchen.de
Organization name LMU Munich
Street address GroƟhaderner Strasse 9
City Planegg
ZIP/Postal code 82152
Country Germany
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (33)
GSM3815033 GS277_RPM
GSM3815034 GS285_RPM
GSM3815035 GS293_RPM
Relations
BioProject PRJNA544681
SRA SRP199438

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE131751_RAW.tar 19.7 Mb (http)(custom) TAR (of TAB, TXT)
GSE131751_TPM_all_samples_values.csv.gz 1.1 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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