|
| Status |
Public on Jan 31, 2020 |
| Title |
BS21_cDC2 |
| Sample type |
SRA |
| |
|
| Source name |
kidney
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: kidney
age: 10 weeks
genotype: Clec9a-Cre/Cre Rosa-YFP/YFP
cell type: cDC2
sorting strategy: CD45+ Ly6C- MHCII+ CD11c+ CD64- CD11b+ YFP+
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA isolation with PicoPure Kit according to protocol. Cells were sorted directly in lysis buffer supplied with the kit
cDNA creation with SMARTseq v4 Kit (Takara), library creation with MicroPlex Library Preparation Kit v2 (Diagenode)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Sequencing reads were aligned to mm10 mouse genome using STAR
Gene expression in transcripts per kilobase million (TPM) was calculated with RSEM
Genome_build: mm10
Supplementary_files_format_and_content: comma separated value file contains TPM values for each sample, *tab files contain read counts.
|
| |
|
| Submission date |
May 24, 2019 |
| Last update date |
Jun 10, 2020 |
| Contact name |
Barbara Schraml |
| E-mail(s) |
barbara.schraml@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Street address |
Großhaderner Strasse 9
|
| City |
Planegg |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE131751 |
RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice |
|
| Relations |
| BioSample |
SAMN11845945 |
| SRA |
SRX5892966 |
