|
Status |
Public on Mar 01, 2010 |
Title |
Pool9 |
Sample type |
RNA |
|
|
Source name |
hypothalamus
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J genotype: wild-type age: P5 fasting: No pool: 9 sample id: EW
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocols.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1
|
Data processing |
The data were normalised using quantile normalisation in R
|
|
|
Submission date |
Feb 25, 2009 |
Last update date |
Mar 03, 2011 |
Contact name |
Jun Li |
E-mail(s) |
junzli@umich.edu
|
Phone |
734-615-5754
|
Organization name |
University of Michigan
|
Street address |
5789A Medical Science II, Box 5618
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL6103 |
Series (1) |
GSE14984 |
Neonatal starvation response and developmental changes in gene expression revealed by hypothalamic expression profiling |
|