|
| Status |
Public on Jul 15, 2019 |
| Title |
DKO mice Lung sample No. G186.1A |
| Sample type |
RNA |
| |
|
| Source name |
DKO Lung 4
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lung
developmental stage: postnatal day 0
genotype/variation: HRAS and NRAS-deficient
|
| Treatment protocol |
Pregnant females were subcutaneously injected twice with 0.4mg/kg of dexamethasone at embryonic stages 17.5 and 18.5. (Only in SAMPLES 15, 16 and 17)
|
| Growth protocol |
Animals were housed in type IIL individually ventilated cages, in a temperature- and humidity-controlled room with a 12h light/dark cycle, with food (Teklad 2014, Harlan Laboratories) and water available ad libitum. All the lung samples were collected from postnatal day 0 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Manually selected lungs of Control, HRas-KO, NRas-KO and DKO from postnatal day 0 were used for RNA extraction using the TRIZOL protocol. RNA was further purified using Qiagen Rneasy mini kit. Each RNA sample for each microarray was obtained from one individual animal.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Ambion)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450)
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Gene expression data from whole HRAS and NRAS-deficient lung extracts.
Lung tissue obtained from postnatal day 0 mice
|
| Data processing |
The RMA function provided by the package Affy from R/Bioconductor was applied to the raw expression data.
|
| |
|
| Submission date |
Apr 29, 2019 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Eugenio Santos |
| E-mail(s) |
esantos@usal.es
|
| Phone |
+34923294801
|
| Organization name |
Universidad de Salamanca y CBERONC
|
| Department |
Instituto de Biología Celular y Molecular del Cáncer
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| ZIP/Postal code |
E37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE130415 |
Transcriptional profiling reveals an upregulation of genes related to Sphingolipid metabolism and Oxidative phosphorilation in HRas and NRas double-KO mice (DKO), which die because of a severe respiratory distress. |
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