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| Status |
Public on Jul 15, 2019 |
| Title |
Transcriptional profiling reveals an upregulation of genes related to Sphingolipid metabolism and Oxidative phosphorilation in HRas and NRas double-KO mice (DKO), which die because of a severe respiratory distress. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used microarrays to investigate gene expression changes in the lungs of Control, Hras-KO, Nras-KO and DKO mice. These DKO animals die at birth because of respiratory distress, caused by an impaired lung maturation.
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| Overall design |
Manually selected lungs of Control, HRas-KO, NRas-KO and DKO from postnatal day 0 were used for RNA extraction using the TRIZOL protocol. RNA was further purified using Qiagen Rneasy mini kit. Each RNA sample for each microarray was obtained from one individual animal.
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| Contributor(s) |
Fuentes-Mateos R, Fernandez-Medarde A, Santos E |
| Citation(s) |
31685810 |
| |
| Submission date |
Apr 29, 2019 |
| Last update date |
Oct 19, 2021 |
| Contact name |
Eugenio Santos |
| E-mail(s) |
esantos@usal.es
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| Phone |
+34923294801
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| Organization name |
Universidad de Salamanca y CBERONC
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| Department |
Instituto de Biología Celular y Molecular del Cáncer
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| Street address |
Campus Miguel de Unamuno
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| City |
Salamanca |
| ZIP/Postal code |
E37007 |
| Country |
Spain |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA540213 |
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