|
| Status |
Public on Feb 10, 2009 |
| Title |
Normal NO076 |
| Sample type |
RNA |
| |
|
| Source name |
Homo Sapiens
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Normal
tissue: kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
R-Phycoerythrin Streptavidin (SAPE), from Molecular Probes, 1 mg/ml ( we use 6 ul per reaction )
|
| Label protocol |
10 ug of Qiagen-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression data from human normal kidney tissue and human kidney tumor tissues.
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - The version 6 entrez gene ID CDF was used.
|
| |
|
| Submission date |
Feb 09, 2009 |
| Last update date |
Feb 09, 2009 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL4866 |
| Series (1) |
| GSE14762 |
Renal Cell Carcinoma: Hypoxia and Endocytosis |
|
