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| Status |
Public on Mar 01, 2020 |
| Title |
HS_2 |
| Sample type |
SRA |
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| Source name |
human embryonic stem cells
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| Organism |
Homo sapiens |
| Characteristics |
tissue: human embryonic stem cells
cell type: hepatic specification
passage: 0
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| Extracted molecule |
total RNA |
| Extraction protocol |
Adherent cells and suspension cells were extracted from the blood of tissue samples ,then add lysate TRNzol-A+ ,after lysis ,centrifugation with chloroform ,RNA was extracted from supernatant
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
bcl2fastq2-v2.19.1 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to GRCh38.91 whole genome using hisat2-2.1.0 with default parameters
Reads count was calculated by htseq-count with parameters -t exon -f bam -s no -a 10
Genome_build: GRCh38.91
Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each Sample
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| Submission date |
Mar 22, 2019 |
| Last update date |
Mar 01, 2020 |
| Contact name |
wang chuan wen |
| E-mail(s) |
729360943@qq.com
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| Organization name |
AnnoGene
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| Street address |
No. 288, Yang Cun road, Chou Jiang Street, Yiwu, Zhejiang
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| City |
YiWu |
| ZIP/Postal code |
322000 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (1) |
| GSE128717 |
Human Embryonic Stem Cell-derived Expandable Hepatic Organoids Enable Pathophysiological Model of Alcoholic Liver Injury |
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| Relations |
| BioSample |
SAMN11233436 |
| SRA |
SRX5559875 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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