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Series GSE128717 Query DataSets for GSE128717
Status Public on Mar 01, 2020
Title Human Embryonic Stem Cell-derived Expandable Hepatic Organoids Enable Pathophysiological Model of Alcoholic Liver Injury
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the generation of human pluripotent-stem-cell-derived (hPSC), expandable hepatic organoids (hEHOs) using a newly established method that consists of subjecting hPSCs to a sequence of distinct wholly defined (serum-free, feeder free) media lineage restricting the cells to become determined hepatic stem cells followed by a process of shifting the cells from monolayer (2D) to organoid (3D ) cultures. The hEHOs stably keep phenotypic features of a bi-potent hepatic lineage that can differentiate into functional hepatocytes or cholangiocytes. The hEHOs can expand for over 20 passages enabling industrial scaling to amounts requisite for industry or clinical programs. The cells from culture are able to engraft rapidly into injured liver parenchyma of FRG mice following transplantation and to differentiate in vivo into mature hepatocytes. If implanted into the epididymis fat pads of immune-deficiency mice, they do not generate non-hepatic lineages nor teratomas. We further developed a derivative model by incorporating human fetal liver mesenchymal cells (hFLMCs) into the hEHOs, referred as hFLMC/hEHO, and used the organoids to model alcohol liver disease-associated pathophysiologic changes, such as oxidative stress generation, steatosis, inflammatory mediators release and fibrosis, following treatment with alcohol. Our work demonstrates that the hFLMC/hEHO provide a novel ex vivo pathophysiological model for studying alcohol liver disease as well as many other non-genetic liver diseases.
Overall design evaluation of whole transcriptome of 7 cell types, each of them include 2 replication
Contributor(s) Wang Y, Wang S
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Submission date Mar 22, 2019
Last update date Mar 01, 2020
Contact name wang chuan wen
Organization name AnnoGene
Street address No. 288, Yang Cun road, Chou Jiang Street, Yiwu, Zhejiang
City YiWu
ZIP/Postal code 322000
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (14)
GSM3683639 FSCs_1
GSM3683640 FSCs_2
GSM3683641 HS_1
BioProject PRJNA528629
SRA SRP189161

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Supplementary file Size Download File type/resource
GSE128717_fpkm.txt.gz 3.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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