|
| Status |
Public on Jan 28, 2019 |
| Title |
HCT116_ARID1A-WT_APR-246_24hr_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HCT116, ARID1A-WT, APR-246, 24hr, replicate2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
genotype: ARID1A-WT
|
| Treatment protocol |
ARI1DA-WT and ARID1A-KO HCT116 cancer cells were treated with 0 or 40 μM APR-246. After 24 h, total RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy kit.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.25 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
cDNA was hybridized to Agilent microarrays (SurePrint G3 Human Gene Expression 8 x 60K Ver.1.0, G4851: 42405 probes) using a Gene Expression Hybridization Kit (Agilent Technologies) for 16 h at 65°C in duplicate.
|
| Scan protocol |
After the arrays were washed using the Gene Expression Wash Pack (Agilent Technologies), the data were extracted using an Agilent scanner.
|
| Description |
Gene expression after 24hr in 40 μM APR-246 treated cells
|
| Data processing |
The arrays were first analyzed using the Feature Extraction software (Agilent Technologies).
|
| |
|
| Submission date |
Nov 26, 2018 |
| Last update date |
Jan 28, 2019 |
| Contact name |
Takashi Kohno |
| E-mail(s) |
tkkohno@ncc.go.jp
|
| Phone |
+81-3-3547-2511 (4650)
|
| Organization name |
National Cancer Center Research Institute
|
| Lab |
Division of Genome Biology
|
| Street address |
1-1, Tsukiji 5-chome
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE122925 |
Genome-wide gene expression analysis in treatment for 24 h with 40 μM APR-246 in ARID1A-WT and ARID1A-KO HCT116 cells. |
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