|
| Status |
Public on Jun 01, 2019 |
| Title |
E-PT_2cell.1 |
| Sample type |
SRA |
| |
|
| Source name |
E-PT_2cell
|
| Organism |
Mus musculus |
| Characteristics |
strain background: BDF1xICR
genotype/variation: Zar1-/+
developmental stage: 2-cell embyro
tissue: early pronuclear transfer 2 cell embryo
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Zar-/+ early pronuclear transfer 2cell embryos
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Low quality base and adapters were removed by cutadapt v1.11
Sequenced reads were mapped to mm9 using HISAT2 version 2.1.0 with default parameters
Gene expressions were calculated by cufflinks v2.2.1
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include FPKM values
|
| |
|
| Submission date |
Nov 05, 2018 |
| Last update date |
Jun 02, 2019 |
| Contact name |
Jin Sun |
| E-mail(s) |
sunjin@tongji.edu.cn
|
| Organization name |
Tongji University
|
| Department |
life science and technology
|
| Lab |
Jiang
|
| Street address |
siping1239
|
| City |
shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE122131 |
Genome transfer for the prevention of female infertility caused by maternal gene mutation |
|
| Relations |
| BioSample |
SAMN10374638 |
| SRA |
SRX4977350 |
