|
| Status |
Public on Feb 26, 2019 |
| Title |
RNA_WT_PgAT_Male_3 [3802WT_PgAT] |
| Sample type |
SRA |
| |
|
| Source name |
perigonadal adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Bcl6 fl/fl
Sex: male
tissue: perigonadal adipose tissue
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was isolated from adipose tissues using Trizol (ThermoFisher) and Rneasy columns (Qiagen). RNA quality was assessed using a Bioanalyzer (Agilent).
TruSeq Stranded mRNA sample prep kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
male wild type perigonadal adipose tissue mRNA
|
| Data processing |
Alignment: Sequenced reads were aligned to the mm10 reference mouse genome using STAR
Differenial Expression Analysis: differentially expressed RNAs were identified using DESeq2 (FDR-adjusted p-value <0.05). STAR alignment and DESeq2 analysis were executed through the RNA Express BaseSpace application (Illumina).
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text (txt) file with differential expression
|
| |
|
| Submission date |
Oct 16, 2018 |
| Last update date |
Feb 26, 2019 |
| Contact name |
Grant D. Barish |
| Organization name |
Northwestern University
|
| Department |
Medicine
|
| Street address |
303 E Superior Street
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE113406 |
Ablation of adipocyte BCL6 enhances subcutaneous adiposity and insulin sensitivity [RNA-seq] |
| GSE113408 |
Ablation of adipocyte BCL6 enhances subcutaneous adiposity and insulin sensitivity |
|
| Relations |
| BioSample |
SAMN10246455 |
| SRA |
SRX4892074 |
