|
| Status |
Public on Nov 08, 2008 |
| Title |
Ag-P-CEN.PK-1N-B-g217-c2-RNA |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
F24 1/13/6 RNA (Strain Name : CEN.PK haploid)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain Name : CEN.PK haploid
|
| Biomaterial provider |
Maitreya Dunham, Dunham Lab, University of Washington
|
| Growth protocol |
Media : phosphate-limited chemostat media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted molecule : Total RNA
Extraction method : acid phenol prep http://genomics.princeton.edu/dunham/MDyeastRNA.pdf
|
| Label |
Cy3
|
| Label protocol |
reference URL : http://genomics.princeton.edu/dunham/MDAgilentRNA.pdf
|
| |
|
| Channel 2 |
| Source name |
F20 3/3/6 RNA (Strain Name : P6 c2)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain Name : P6 c2
|
| Biomaterial provider |
Maitreya Dunham, Dunham Lab, University of Washington
|
| Growth protocol |
Media : phosphate-limited chemostat media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted molecule : Total RNA
Extraction method : acid phenol prep http://genomics.princeton.edu/dunham/MDyeastRNA.pdf
|
| Label |
Cy5
|
| Label protocol |
reference URL : http://genomics.princeton.edu/dunham/MDAgilentRNA.pdf
|
| |
|
| |
| Hybridization protocol |
reference URL : http://genomics.princeton.edu/dunham/MDAgilentRNA.pdf
|
| Scan protocol |
Pixel Size : 10
Scan Date : 2006-04-05
Scan Time : 11:11:46
Scanner Make : Agilent Technologies Scanner
Scanner Model : G2505B
Scanning software : ChipScan
Scanning software version : A.6.3.1
|
| Description |
Experiment design: RNA from each evolved clone or population grown in chemostat culture is compared to RNA from matching ancestor strains grown in the same conditions
Complete protocols also available as supplementary files on the Series record
|
| Data processing |
Extraction Software : Agilent Feature Extractor
Extraction Software Version : A.7.5.1
Datafile type : Agilent result file
|
| |
|
| Submission date |
Nov 03, 2008 |
| Last update date |
Nov 07, 2008 |
| Contact name |
Maitreya J. Dunham |
| E-mail(s) |
maitreya@uw.edu
|
| Phone |
206-543-2338
|
| Organization name |
University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Dunham Lab
|
| Street address |
Foege Building, S403B, Box 355065
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-5065 |
| Country |
USA |
| |
|
| Platform ID |
GPL2883 |
| Series (1) |
| GSE13435 |
The Repertoire and Dynamics of Evolutionary Adaptations to Controlled Nutrient-Limited Environments in Yeast |
|
