|
| Status |
Public on Apr 28, 2009 |
| Title |
EZH2 RNAi, replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
SKBr3 cells, EZH2 RNAi
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKBr3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
According to the manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
According to the manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
SKBr3 cells, EZH2 control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKBr3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
According to the manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
According to the manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
According to the manufacturer's protocol.
|
| Scan protocol |
Scanned on Agilent Technologies Scanner G2505B.
Images were quantified using Agilent Feature Extraction Software.
|
| Description |
EZH2 RNAi vs. EZH2 control siRNA
Replicate 2 of 2
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Oct 20, 2008 |
| Last update date |
May 26, 2010 |
| Contact name |
Christopher Maher |
| E-mail(s) |
chrmaher@med.umich.edu
|
| Phone |
734-764-3336
|
| Organization name |
Michigan Center for Translational Pathology
|
| Street address |
1500 E. Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48105 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE13286 |
miR101 overexpression vs. EZH2 RNAi |
|
