|
| Status |
Public on Dec 04, 2018 |
| Title |
32h Replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
32h, HPAF-II xenograft - mouse pancreas
|
| Organism |
Homo sapiens |
| Characteristics |
host: mouse
rin: 6.9
timepoint: 32h
cell type: HPAF-II
treatment: ETC-159 (PORCN inhbitor)
|
| Treatment protocol |
Treatment with ETC-159 bid
|
| Growth protocol |
HPAF-II cells injected into the mouse pancreas. Tumors were allowed to develop for 28 days before start of treatment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNAeasy kit (Qiagen)
Illumina TruSeq Stranded polyA enrichment
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
Human graft / mouse host
|
| Data processing |
adaptor trimming using Cutadapt
filtering using Xenome
alignment using STAR - hg38 Ensembl 79
quantification using STAR - hg38 Ensembl 79
Genome_build: hg38 / Ensembl 79
Supplementary_files_format_and_content: RSEM quantification of human reads
|
| |
|
| Submission date |
Aug 02, 2018 |
| Last update date |
Dec 04, 2018 |
| Contact name |
NATHAN HARMSTON |
| E-mail(s) |
harmstonn@cardiff.ac.uk
|
| Organization name |
Cardiff University
|
| Street address |
Museum Avenue
|
| City |
Cardiff |
| ZIP/Postal code |
CF10 3AX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE118041 |
Transcriptional response to PORCN inhibition using an orthotopic model of RNF43 mutant pancreatic adenocarcinoma (HPAF-II) |
|
| Relations |
| SRA |
SRX3875374 |
| BioSample |
SAMN08848854 |
