|
| Status |
Public on Jul 17, 2019 |
| Title |
SMM at diagnosis, sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
Highly purified PCs from MM patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
cell type: plasma cells
patient diagnosis: progressed SMM
|
| Treatment protocol |
Plasma cells were purified from bone marrow samples using CD138 immunomagnetic microbeads according to the manufacturer's instructions (MidiMACS system, Miltenyi Biotec); the purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Gibco BRL).
|
| Label |
biotin
|
| Label protocol |
Biotinylated single stranded cDNA samples were prepared starting from 100 nanograms of total RNA, according to the Affymetrix WT Plus Expression Protocol.
|
| |
|
| Hybridization protocol |
The fragmented labeled single-stranded DNA target was hybridized for 16 hours and 30 minutes at 45°C on GeneChip ClariomD array, according to the standard Affymetrix protocol, using the GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanning of the arrays was performed using the GeneChip Scanner 7G.
|
| Description |
progressed SMM
|
| Data processing |
Normalized expression values were obtained using Robust Multi Array Average (RMA) procedure. The custom CDF GENECODEG version 22 was used from BrainArray.
|
| |
|
| Submission date |
Jul 30, 2018 |
| Last update date |
Jul 17, 2019 |
| Contact name |
Luca Agnelli |
| E-mail(s) |
luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
|
| Phone |
+390223903581
|
| Organization name |
IRCCS Istituto Nazionale dei Tumori
|
| Department |
Department of Advanced Diagnostics
|
| Street address |
Venezian 1
|
| City |
MILAN |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL25401 |
| Series (1) |
| GSE117847 |
A retained transcriptomic profile characterizes the CD138+ cells in the progression from smoldering to active multiple myeloma |
|
