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Status |
Public on Jul 17, 2019 |
Title |
SMM at diagnosis, sample 9 |
Sample type |
RNA |
|
|
Source name |
Highly purified PCs from MM patient
|
Organism |
Homo sapiens |
Characteristics |
tissue: Bone marrow cell type: plasma cells patient diagnosis: progressed SMM
|
Treatment protocol |
Plasma cells were purified from bone marrow samples using CD138 immunomagnetic microbeads according to the manufacturer's instructions (MidiMACS system, Miltenyi Biotec); the purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Gibco BRL).
|
Label |
biotin
|
Label protocol |
Biotinylated single stranded cDNA samples were prepared starting from 100 nanograms of total RNA, according to the Affymetrix WT Plus Expression Protocol.
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|
|
Hybridization protocol |
The fragmented labeled single-stranded DNA target was hybridized for 16 hours and 30 minutes at 45°C on GeneChip ClariomD array, according to the standard Affymetrix protocol, using the GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
Scanning of the arrays was performed using the GeneChip Scanner 7G.
|
Description |
progressed SMM
|
Data processing |
Normalized expression values were obtained using Robust Multi Array Average (RMA) procedure. The custom CDF GENECODEG version 22 was used from BrainArray.
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|
|
Submission date |
Jul 30, 2018 |
Last update date |
Jul 17, 2019 |
Contact name |
Luca Agnelli |
E-mail(s) |
luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
|
Phone |
+390223903581
|
Organization name |
IRCCS Istituto Nazionale dei Tumori
|
Department |
Department of Advanced Diagnostics
|
Street address |
Venezian 1
|
City |
MILAN |
ZIP/Postal code |
20133 |
Country |
Italy |
|
|
Platform ID |
GPL25401 |
Series (1) |
GSE117847 |
A retained transcriptomic profile characterizes the CD138+ cells in the progression from smoldering to active multiple myeloma |
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