|
| Status |
Public on Jan 15, 2019 |
| Title |
Wounded_leaf |
| Sample type |
SRA |
| |
|
| Source name |
leaf
|
| Organism |
Ipomoea batatas |
| Characteristics |
genotype: Wild-type
cultivar: Tainung 57
tissue: leaf
|
| Treatment protocol |
The third fully expanded sweet potato leaves counted from terminal bud were treated by wounding 30 min.
|
| Growth protocol |
Sweet potato was grown in a controlled environment at 25°C under 16 h light/8 h dark with cool fluorescent light at 20 μmol photons m-2 s-1.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol® Reagent (Invitrogen, USA) according to the instruction manual.
Library construction of all samples were used by Illumina Genome Analyzer IIx platform and the 50 bp Single-End Reads
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
Raw sequences were analysis by Mir pipeline on Linux
Clean up poly ATCG
Qualified reads after filtering the low-quality data
Qualified reads after filtering the size (select 18-25)
Conserved miRNA were match with miRBase
Genome_build: miRBase
Supplementary_files_format_and_content: txt file include read counts for sequences.
|
| |
|
| Submission date |
May 31, 2018 |
| Last update date |
Jan 15, 2019 |
| Contact name |
Yun-Wei Kuo |
| Organization name |
National Taiwan University
|
| Department |
Plant Biology
|
| Street address |
No. 1, Sec. 4, Roosevelt Road
|
| City |
Taipei |
| ZIP/Postal code |
10617 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL17085 |
| Series (1) |
| GSE115176 |
Small RNAs deep sequencing from sweet potato in wounding stress |
|
| Relations |
| BioSample |
SAMN09289690 |
| SRA |
SRX4148929 |
