|
| Status |
Public on Dec 23, 2019 |
| Title |
C57BL/6J-mtBPL/J 2 |
| Sample type |
SRA |
| |
|
| Source name |
Liver obtained from C57BL/6J-mtBPL/J mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J-mtBPL/J
tissue: Liver
mutation: mt-Nd5 m.11902 T>C
Sex: female
age: 3 months
replicate: replicate 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted from liver samples obtained from conplastic mouse strains (C57BL/6J and C57BL/6J-mtAKR/J), using innuPREP RNA Mini Kit (Analytik Jena) according to the manufacturer’s protocol. RNAs quality was checked using a Bioanalyzer system (Agilent Genomics).
RNA sequencing of primary samples was carried out by using oligo-dT beads to enrich molecules with poly-A tails. Libraries were sequenced on a Illumina HiSeq 2500 as 75bp single ended.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
B6_mtBPL_rep2
|
| Data processing |
Raw sequence reads were pseudo aligned to the mouse transcriptome mm10 (Ensembl Build 84) using Kallisto (Version 0.44.0, Bray et al. Nat. Biotechnology 2016) runing 30 bootstrap rounds.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text files per sample include effectice read counts and transcript per million (TPM) values for each transcript
|
| |
|
| Submission date |
May 09, 2018 |
| Last update date |
Dec 24, 2019 |
| Contact name |
Hauke Busch |
| E-mail(s) |
hauke.busch@uni-luebeck.de
|
| Phone |
+49-451-3101-8470
|
| Organization name |
University of Lübeck
|
| Department |
Lübeck Institute of Experimental Dermatology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23538 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE114256 |
Polymorphisms in mitochondrial DNA genes coding for subunits in complex I differentially control lifespan and ageing-related pathways in mice. |
|
| Relations |
| BioSample |
SAMN09099474 |
| SRA |
SRX4061229 |
