|
| Status |
Public on Aug 25, 2009 |
| Title |
P28/10mg/kg/W0 |
| Sample type |
RNA |
| |
|
| Source name |
colonic biopsy; WK8RSPHM: No
|
| Organism |
Homo sapiens |
| Characteristics |
WK8RSPHM: No
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen's RNeasy mini kit according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 11 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Response to infliximab was defined as endoscopic and histologic healing at week 8
|
| Data processing |
The data were analyzed with GCOS 1.4 (GeneChip Operating System) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Jul 25, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
K Li |
| E-mail(s) |
kli@its.jnj.com
|
| Organization name |
Janssen R&D
|
| Street address |
1400 McKean Road
|
| City |
Spring House |
| State/province |
PA |
| ZIP/Postal code |
19477 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12251 |
A Predictive Response Signature to Infliximab Treatment in Ulcerative Colitis |
|
| Relations |
| Reanalyzed by |
GSE119087 |
