|
| Status |
Public on Jul 07, 2009 |
| Title |
Bt_DE18 |
| Sample type |
RNA |
| |
|
| Source name |
Male beef cattle, TREATED WITH DEXAMETHAZONE via feed 0.75 mg/head for 43 days and WITH 17β-ESTRADIOL intramuscolarly 20mg/head for three times
|
| Organism |
Bos taurus |
| Characteristics |
Anterior limb muscle (biceps branchii)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted according to QiAgen RNeasy MiniKit protocol for tissues samples
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Anterior limb muscle collected from male beef cattle, submerged in RNALater and stored at -20°C until extraction.
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using R2.6.1 statistical software
|
| |
|
| Submission date |
Jul 21, 2008 |
| Last update date |
Jul 07, 2009 |
| Contact name |
Serena Ferraresso |
| E-mail(s) |
serena.ferraresso@unipd.it
|
| Phone |
+39 049 8272506
|
| Organization name |
University of Padova
|
| Department |
Dept of Comparative Biomedicine and Food Science
|
| Street address |
Viale dell'Università
|
| City |
Legnaro (PD) |
| State/province |
Padova |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7053 |
| Series (1) |
| GSE12179 |
Expression profiling of skeletal muscle in beef cattle treated with steroidal growth promoters |
|
