|
Status |
Public on Jul 07, 2009 |
Title |
Bt_DE16 |
Sample type |
RNA |
|
|
Source name |
Male beef cattle, TREATED WITH DEXAMETHAZONE via feed 0.75 mg/head for 43 days and WITH 17β-ESTRADIOL intramuscolarly 20mg/head for three times
|
Organism |
Bos taurus |
Characteristics |
Anterior limb muscle (biceps branchii)
|
Extracted molecule |
total RNA |
Extraction protocol |
Extracted according to QiAgen RNeasy MiniKit protocol for tissues samples
|
Label |
Cyanine 3
|
Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
|
|
Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
Description |
Anterior limb muscle collected from male beef cattle, submerged in RNALater and stored at -20°C until extraction.
|
Data processing |
Data processed with FeatureExtraction v9.5 and normalized using R2.6.1 statistical software
|
|
|
Submission date |
Jul 21, 2008 |
Last update date |
Jul 07, 2009 |
Contact name |
Serena Ferraresso |
E-mail(s) |
serena.ferraresso@unipd.it
|
Phone |
+39 049 8272506
|
Organization name |
University of Padova
|
Department |
Dept of Comparative Biomedicine and Food Science
|
Street address |
Viale dell'Università
|
City |
Legnaro (PD) |
State/province |
Padova |
ZIP/Postal code |
35020 |
Country |
Italy |
|
|
Platform ID |
GPL7053 |
Series (1) |
GSE12179 |
Expression profiling of skeletal muscle in beef cattle treated with steroidal growth promoters |
|