|
| Status |
Public on Nov 30, 2008 |
| Title |
Ad-F7 transduction + anti-IFNAR2 treatment in primary human macrophages (Experiment 2) |
| Sample type |
RNA |
| |
|
| Source name |
Macrophages Ad-F7 + anti-IFNAR2
|
| Organism |
Homo sapiens |
| Characteristics |
Human macrophages transduced with adenoviral vector expression the active form of IRF-7 (IRF-7 D247-467; Ad-F7) and treatment of cells with neutralizing antibody for the type I IFN receptor (IFNAR2). RNA collected at 24 hours.
|
| Treatment protocol |
Transduction of primary human macrophages with Ad-F7 and treatment of cells with anti-IFNAR2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus extraction kit
|
| Label |
Cy3
|
| Label protocol |
Ambion, TotalPrep, for biotin labeling and illumina's protocol for Cy3 labeling.
|
| |
|
| Hybridization protocol |
From illumina
|
| Scan protocol |
From illumina
|
| Description |
See illumina's sample preparation
|
| Data processing |
BeadStudio from illumina, J-Express from Molmine and R
|
| |
|
| Submission date |
Jul 03, 2008 |
| Last update date |
Sep 24, 2008 |
| Contact name |
Eduardo Hernandez |
| E-mail(s) |
camacho99@yahoo.com
|
| Organization name |
Lady Davis Institute for Medical Research (JGH)
|
| Department |
Molecular Oncology Group
|
| Lab |
526
|
| Street address |
3755 Cote-Sainte-Catherine Road
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T-1E2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6883 |
| Series (2) |
| GSE12002 |
Transcriptional profiles of Ad-F7 transduced macrophages treated with anti-IFNAR2 antibody or control isotype (IgG) |
| GSE12120 |
Transcriptional re-programming of primary human macrophages by IRF-3 and IRF-7 |
|
