|
| Status |
Public on Dec 31, 2018 |
| Title |
IFT88KD Myoblasts,Proliferating condition,Replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
IFT88 siRNA treated C2C12 myoblasts in adherant culture with growth medium
|
| Organism |
Mus musculus |
| Characteristics |
cell type: skeletal muscle myoblasts
|
| Treatment protocol |
Cells collected from different conditions were washed in ice cold PBS and lysed in TRIZol solution
|
| Growth protocol |
Control and IFT88KD C2C12 myoblasts were cultured in different conditions to achieve different cell cycle states (Proliferating myoblasts (MB):Adherent culture, full growth medium (DMEM+20% FBS); Quiescent myoblasts (G0): Suspension culture, full growth medium (DMEM+20% FBS); Reactivated myoblasts (R2): G0 myoblasts replated after suspenion culture, full growth medium (DMEM+20% FBS); Differentiating myoblasts (D48): 80% confluent myoblasts in adherent culture cultured in Differentiation medium (DMEM +2% NHS) for 48 hours.)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChipMouse Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
All the CEL (cell intensity) files generated by the Affymetrix Gene Chip operating software (GCOS) Expression Console were loaded into the R Bioconductor affy package for microarray analysis,normalized using Loess Normalization method
|
| |
|
| Submission date |
Feb 16, 2018 |
| Last update date |
Dec 31, 2018 |
| Contact name |
Nisha Venugopal |
| Organization name |
CCMB
|
| Street address |
Habsiguda, Uppal Road
|
| City |
Hyderabad |
| State/province |
Telangana |
| ZIP/Postal code |
500007 |
| Country |
India |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE110742 |
Effect of loss of ciliogenesis on myoblast quiescence |
|
