|
| Status |
Public on Nov 22, 2019 |
| Title |
FetalTreg2 |
| Sample type |
SRA |
| |
|
| Source name |
Fetal spleenocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: fetal Treg cells
tissue: Fetal spleenocytes
age: 22.2 weeks
Sex: Not determined
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted by FACS, and total RNA was extracted from 250,000 cells using the Nucleospin RNA kit (Machery Nagel)
Libraries were constructed using a standard Illumina protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Description |
FT2
|
| Data processing |
Fastq files were mapped to the genome using Bowtie 2
Read counts per gene was obtained using RSEM
TMM normalization and differential expression analysis performed using EdgeR package for Bioconductor
Genome_build: hg19
Supplementary_files_format_and_content: TMM-normalized data.
|
| |
|
| Submission date |
Feb 12, 2018 |
| Last update date |
Nov 24, 2019 |
| Contact name |
Melissa Ng |
| E-mail(s) |
melissa.ng@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Lab |
Burt Lab
|
| Street address |
35 Medical Centre Way, Regeneration Medicine Building 903B
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (2) |
| GSE110469 |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. [RNA-Seq - ex vivo] |
| GSE110472 |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. |
|
| Relations |
| BioSample |
SAMN08518234 |
| SRA |
SRX3680691 |
