|
| Status |
Public on Nov 22, 2019 |
| Title |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. [RNA-Seq - ex vivo] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing was used to compare the transcriptome of ex vivo isolated adult and fetal naïve and regulatory T (Treg) cells. A Treg-specific transcriptome was defined by genes upregulated and downregulated preferentially in adult and fetal Treg cells relative to adult naive T cells. Fetal naive T cells show an intermediate expression of genes in the Treg-specific transcriptome.
|
| |
|
| Overall design |
RNA sequencing for 4 biological replicates of ex vivo sorted CD4+CD25-CD45RA+CD27+ naïve and CD4+CD25hiCD127lo Treg cells from fetal spleen and adult peripheral blood mononuclear cells.
|
| |
|
| Contributor(s) |
Ng MS, Burt TD |
| Citation(s) |
31757834 |
| Submission date |
Feb 12, 2018 |
| Last update date |
Feb 21, 2020 |
| Contact name |
Melissa Ng |
| E-mail(s) |
melissa.ng@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Lab |
Burt Lab
|
| Street address |
35 Medical Centre Way, Regeneration Medicine Building 903B
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE110472 |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. |
|
| Relations |
| BioProject |
PRJNA433825 |
| SRA |
SRP132689 |
Less...
More...