|
| Status |
Public on Feb 10, 2018 |
| Title |
Inguinal WAT, thermoneutrality, wild type mouse, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Inguinal white adipose tissue from a wild type mouse housed at thermoneutrality (28 °C) for 10 days
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
genotype: wild type
jackson laboratories mouse strain id: 664
housing temperature: thermoneutrality (28 C) for 10 days
Sex: male
|
| Treatment protocol |
Forty 3-4-month-old male wildtype and PPARα−/− mice were placed at thermo-neutral temperature (28 °C) for 5 weeks. Thereafter, 20 wildtype and 20 PPARα−/− mice were randomly distributed across 2 groups: half of the mice of each genotype were kept at thermo-neutral temperature and half of the mice of each genotype was placed at 21 °C for one week followed by a period of 10 days at in the cold (5 °C) (n = 10 per group). For cold exposure, mouse cages were placed in a cold cabinet that was kept at 5 °C (ELDG800, VDW Coolsystems, Geldermalsen). Mice had ad libitum access to chow feed and water.
|
| Growth protocol |
Three to four months old male wildtype and PPARα−/− mice that had been backcrossed on a pure C57Bl/6J background for more than 10 generations were acquired from Jackson Laboratories (no. 000664 and 008154, respectively). The mice were further bred at the animal facility of Wageningen University under specific pathogen free conditions to generate the number of mice necessary for the experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy micro kit from Qiagen. RNA integrity was checked on chip analysis (Agilent 2100 bioanalyzer, Agilent Technologies, Amsterdam, the Netherlands) according to the manufacturer's instructions. RNA was judged as suitable for array hybridization only if samples exhibited intact bands corresponding to the 18S and 28S ribosomal RNA subunits, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0).
|
| Label |
biotin
|
| Label protocol |
Purified total RNA (100ng per sample) was labeled with the Whole-Transcript Sense Target Assay (Affymetrix, Santa Clara, CA, USA; P/N 900652).
|
| |
|
| Hybridization protocol |
Hybridization and washing of the Affymetrix GeneChip Mouse Gene 2.1 ST peg arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).
|
| Data processing |
Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.42.0).
|
| |
|
| Submission date |
Feb 09, 2018 |
| Last update date |
Feb 10, 2018 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE110420 |
The Peroxisome Proliferator-Activated Receptor α is dispensable for cold-induced adipose tissue browning in mice |
|
