|
| Status |
Public on Sep 30, 2018 |
| Title |
Wild type, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Pseudomonas aeruginosa airway infection
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
host strain: C57BL/6
host strain gener: female
infection tissue: mouse lung
bacteria infection: P. aeruginosa in vivo infection
time point: 24 h post-infection
|
| Treatment protocol |
Infected animals as described above were euthanized 24 h post-infection.
|
| Growth protocol |
P.aeruginosa strains were grown in LB at 37°C overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from airway homogenate pellets using Trizol according to the manufacturer's instructions. Samples were treated with DNAse, then rRNA was removed from 1 to 5 μg of total RNA using the RiboZero kit for Gram-negative bacteria. rRNA-depleted samples were concentrated by EtOH precipitation. cDNA synthesis and hybridization to Affymetrix GeneChip P. aeruginosa genome arrays.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip P. aeruginosa Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChip 3000 7G scanner
|
| Description |
WT_1
|
| Data processing |
Biomedical Genomics Workbench 4
|
| |
|
| Submission date |
Feb 05, 2018 |
| Last update date |
Sep 30, 2018 |
| Contact name |
Shi-qi An |
| Organization name |
Queen's University Belfast
|
| Street address |
Lisburn Road
|
| City |
Belfast |
| ZIP/Postal code |
UK BT9 7BL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE110126 |
The impact of DSF and C23 on P. aeruginosa physiology |
|
