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| Status |
Public on Sep 30, 2018 |
| Title |
The impact of DSF and C23 on P. aeruginosa physiology |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
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| Summary |
To verify the imapct of DSF and C23 on P. aeruginosa during infection
We used microarray to compare the effects of adding either DSF alone or DSF with C23 on P. aeruginosa gene expression during mouse lung infection relative to the gene expression of P. aeruginosa in the mouse lung with no compound.
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| Overall design |
For the in vivo RNA samples, infected animals as described above were euthanized 24 h post-infection. RNA was isolated from airway homogenate pellets using Trizol according to the manufacturer's instructions. Samples were treated with DNAse, then rRNA was removed from 1 to 5 μg of total RNA using the RiboZero kit for Gram-negative bacteria (Epicentre). rRNA-depleted samples were concentrated by EtOH precipitation. cDNA synthesis and hybridization to Affymetrix GeneChip P. aeruginosa genome arrays.
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| Contributor(s) |
An S |
| Citation(s) |
31133642 |
| |
| Submission date |
Feb 05, 2018 |
| Last update date |
Jun 18, 2019 |
| Contact name |
Shi-qi An |
| Organization name |
Queen's University Belfast
|
| Street address |
Lisburn Road
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| City |
Belfast |
| ZIP/Postal code |
UK BT9 7BL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA432916 |
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