|
Status |
Public on May 22, 2018 |
Title |
Dazl WT GFP+ RNA-seq S1 |
Sample type |
SRA |
|
|
Source name |
Testes
|
Organism |
Mus musculus |
Characteristics |
genotype: Stra8-iCre+; IRG+; DAZL++ (DAZL WT) strain: CD1xC57BL/6J age: p6
|
Treatment protocol |
Isolations of GFP+ cells was performed using dual fluorescence labeling as previously described (Zagore et al., 2015), except P6 cells were not stained with Hoechst and were collected using either BD Biosciences Aria or iCtye Reflection cytometry instrumentation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Illumina TruSeq/Clontech ultra low input library preparation with Nextera XT indexing was used to generate RNA-Seq libraries
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
FACS sorted GFP+ germ cells RPKM WT1
|
Data processing |
Read mapping and gene expression quantification was performed using Olego and Quantas Genome_build: mm9 Supplementary_files_format_and_content: RPKM
|
|
|
Submission date |
Jan 10, 2018 |
Last update date |
May 22, 2018 |
Contact name |
Donny Licatalosi |
E-mail(s) |
ddl33@case.edu
|
Organization name |
Case Western Reserve University
|
Street address |
2109 Adelbert Rd
|
City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44106 |
Country |
USA |
|
|
Platform ID |
GPL17021 |
Series (2) |
GSE108993 |
Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [GFP cells RNA-Seq] |
GSE108997 |
Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions |
|
Relations |
BioSample |
SAMN08336886 |
SRA |
SRX3547131 |