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Sample GSM2927781 Query DataSets for GSM2927781
Status Public on May 22, 2018
Title Dazl WT GFP+ RNA-seq S1
Sample type SRA
 
Source name Testes
Organism Mus musculus
Characteristics genotype: Stra8-iCre+; IRG+; DAZL++ (DAZL WT)
strain: CD1xC57BL/6J
age: p6
Treatment protocol Isolations of GFP+ cells was performed using dual fluorescence labeling as previously described (Zagore et al., 2015), except P6 cells were not stained with Hoechst and were collected using either BD Biosciences Aria or iCtye Reflection cytometry instrumentation.
Extracted molecule total RNA
Extraction protocol Illumina TruSeq/Clontech ultra low input library preparation with Nextera XT indexing was used to generate RNA-Seq libraries
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description FACS sorted GFP+ germ cells
RPKM WT1
Data processing Read mapping and gene expression quantification was performed using Olego and Quantas
Genome_build: mm9
Supplementary_files_format_and_content: RPKM
 
Submission date Jan 10, 2018
Last update date May 22, 2018
Contact name Donny Licatalosi
E-mail(s) ddl33@case.edu
Organization name Case Western Reserve University
Street address 2109 Adelbert Rd
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
 
Platform ID GPL17021
Series (2)
GSE108993 Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [GFP cells RNA-Seq]
GSE108997 Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions
Relations
BioSample SAMN08336886
SRA SRX3547131

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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