|
| Status |
Public on May 22, 2018 |
| Title |
Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions [GFP cells RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Dazl (deleted in azoospermia like) is a member of the DAZ family of germ cell-restricted RNA binding proteins required for gametogenesis from worm to human. The direct RNA targets and functions of these essential proteins are poorly understood. Here, we generated high-resolution, transcriptome-wide maps of Dazl-RNA interactions in mouse testes. These maps provide important insights into the mechanism of Dazl recruitment to mRNA and reveal Dazl binding to thousands of mRNAs predominantly through sequence-specific interactions near the polyA tail. Using transgenic mice and fluorescence activated cell sorting (FACS), we isolated DAZL knockout germ cells and used RNA-Seq to identify mRNAs sensitive to DAZL-ablation. Intersecting the RNA-Seq and Dazl-RNA interaction datasets revealed that Dazl enhances expression of a subset of directly-bound transcripts, namely mRNAs for a network of essential cell cycle regulatory genes. Collectively, our integrative analysis delineates a Dazl-dependent post-transcriptional gene regulatory program essential for mammalian germ cell maintenance.
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| |
|
| Overall design |
RNA-seq libraries generated Dazl WT and Dazl KO GFP+ germ cells
|
| |
|
| Contributor(s) |
Licatalosi DD, Zagore LL |
| Citation(s) |
30380414 |
| |
| Submission date |
Jan 10, 2018 |
| Last update date |
Dec 05, 2019 |
| Contact name |
Donny Licatalosi |
| E-mail(s) |
ddl33@case.edu
|
| Organization name |
Case Western Reserve University
|
| Street address |
2109 Adelbert Rd
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (5)
|
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| This SubSeries is part of SuperSeries: |
| GSE108997 |
Dazl maintains proliferating germ cells through a network of polyA-proximal mRNA interactions |
|
| Relations |
| BioProject |
PRJNA429355 |
| SRA |
SRP128645 |
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