|
| Status |
Public on Jun 16, 2020 |
| Title |
control_U251_48h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
U251, 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U251
|
| Treatment protocol |
The cells was treated with culcita novaeguineae saponin
|
| Growth protocol |
Human glioma cell lines U251 was cultured in DMEM (Hyclone) supplemented with 10 % FBS (Biowest) at 37 ℃ with 5 % CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 12 ug total RNA (Expression Analysis Technical Manual, 2013, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Primeview human Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 7G.
|
| Description |
Gene expression data from U251 MG cells
|
| Data processing |
The data were analyzed with rma using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 20, 2017 |
| Last update date |
Jun 18, 2020 |
| Contact name |
Peng-Cheng Qiu |
| E-mail(s) |
qpc023@126.com
|
| Phone |
86-13772051376
|
| Organization name |
Fourth Military Medical University
|
| Street address |
Changle West Road 169
|
| City |
Xi'an |
| ZIP/Postal code |
710032 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE108343 |
a New Marine-Derived Asterosaponin (CN-3) alleviated the proliferation of U251 MG cells |
|
