|
| Status |
Public on Sep 04, 2018 |
| Title |
Ter119pos_PRMT5_KO_B |
| Sample type |
SRA |
| |
|
| Source name |
E14.5 Fetal Liver Cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: PRMT5fl/fl; Vav-cre
mouse #: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol (Invitrogen #15596-026).
RNA-Seq library prep was carried out using the Illumina TruSeq Total Stranded kit (RS-122-2201) with Ribo-Zero rRNA reduction following manufacturer’s standard protocol without modification.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
RSEM.gene.results.txt
|
| Data processing |
Base calling using Illumina RTA 2.4.11
Reads mapped to GRCm38/mm10 transcriptome using STAR v2.5.3 and RSEMv1.3.0 with default parameters.
Estimated counts were obtained from RSEM.gene.results
Genome_build: GRCm38
Supplementary_files_format_and_content: Tab-delimited file contains aggragated raw RSEM expected gene level counts for all samples .
|
| |
|
| Submission date |
Dec 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Nimer |
| Organization name |
University of Miami
|
| Department |
Sylvester Comprehensive Cancer Center
|
| Street address |
1120 NW 14th ST
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE107854 |
PRMT5 regulates the homologous recombination DNA repair pathway by controlling the alternative splicing of key epigenetic factors |
|
| Relations |
| BioSample |
SAMN08152676 |
| SRA |
SRX3459451 |
