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| Status |
Public on Sep 04, 2018 |
| Title |
PRMT5 regulates the homologous recombination DNA repair pathway by controlling the alternative splicing of key epigenetic factors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We found that PRMT5 deletion or inhibition impairs homologous recombination (HR) DNA repair, leading to DNA damage accumulation, p53 activation, cell cycle arrest and cell death through missplicing of KAT5.
We show that PRMT5 inhibitors and PARP inhibitors have synergistic effects on human acute leukemia cell lines, demonstrating the advantages of combining targeted epigenetic and non-epigenetic inhibitors.
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| Overall design |
RNA from sorted E14.5 Ter119-/+ mouse fetal liver cells from Vav-cre;PRMT5fl/fl (KO) and PRMT5fl/fl (WT) were subjected to high-throughput RNA sequencing. RNA from sorted LK cells from Mx1-cre;PRMT5fl/fl (KO) and PRMT5fl/fl (WT) collected 7 days after poly(I:C) injections were subjected to high-throughput RNA sequencing.
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| Contributor(s) |
Hamard P, Daniel K |
| Citation(s) |
30184499 |
| |
| Submission date |
Dec 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Nimer |
| Organization name |
University of Miami
|
| Department |
Sylvester Comprehensive Cancer Center
|
| Street address |
1120 NW 14th ST
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA421729 |
| SRA |
SRP126416 |
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