|
| Status |
Public on Jun 06, 2008 |
| Title |
PBMC_WY_personF |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells, WY14,643 treated for 12 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Male, aged between 30-48
|
| Treatment protocol |
PBMCs were incubated in RPMI1640 medium with 2 mmol/L L-glutamine, 10% fetal bovine serum and antibiotics (penicillin and streptomycin) in the presence of 5% CO2 at 37°C. at 1e6 cells per ml with either WY14,643 (50 μM) or vehicle (DMSO, 0.1%) for 12 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were lysed in Qiagen RLT buffer and homogenized using seringes with 0.4 mm needles, whereafter purified total RNA was isolated using Qiagen RNEasy columns
|
| Label |
biotin
|
| Label protocol |
Total RNA from PBMCs (5µg) was labeled using the Affymetrix One-cycle Target Labeling Assay
|
| |
|
| Hybridization protocol |
Arrays were hybridized on an Affymetrix 450 fluidics stations according to the protocol Midi_euk2v3_450, as described by Affymetrix
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip scanner 3000 7G, according to the standard protocols of Affymetrix
|
| Description |
PBMC_WY_personF
|
| Data processing |
Expression estimates were calculated using RMA
|
| |
|
| Submission date |
Apr 29, 2008 |
| Last update date |
Jun 06, 2008 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6791 |
| Series (1) |
| GSE11289 |
Activation of peroxisome proliferator-activated receptor alpha in human peripheral blood mononuclear cells |
|
