|
| Status |
Public on Jun 23, 2008 |
| Title |
Mutant histone H4R45H- replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast RNA, H4 R45H (Arginine 45 of histone H4 mutated to Histidine)
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
H4 R45H (Arginine 45 of histone H4 mutated to Histidine)
|
| Treatment protocol |
Cells were fixed in liquid nitrogen
|
| Growth protocol |
Cells were grown in YPD media until mid log phase and harvested at OD600 of 0.5-0.7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and RNA was extracted by hot acid phenol chloroform and phenol chloroform isoamyl alcohol extraction.
|
| Label |
Biotin
|
| Label protocol |
Approximately 20 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
Mutant yeast strain H4 R45H
|
| Data processing |
Affymetrix Microarray Suite version 5.0.
|
| |
|
| Submission date |
Apr 28, 2008 |
| Last update date |
Jun 23, 2008 |
| Contact name |
Michael J. Smerdon |
| E-mail(s) |
smerdon@wsu.edu
|
| Phone |
509-335-6853
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
Box 644660
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164-4660 |
| Country |
USA |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE11282 |
Comparison of yeast (Saccharomyces cerevisiae) strain with histone H4 R45 mutated to H with wild type yeast strain WY139 |
|
