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| Status |
Public on Jun 23, 2008 |
| Title |
Comparison of yeast (Saccharomyces cerevisiae) strain with histone H4 R45 mutated to H with wild type yeast strain WY139 |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Total RNA samples from three replicate cultures of wild type and mutant yeast strains was isolated and expression profile done using Affymetrix arrays. Comparsion between the samples indicate how mutation in a single amino acid residue in histone H4 (H4R45H) affects gene expression in yeast. Such a mutation in histone H4 is known to generate a specific class of mutants called SWI/SNF independent (SIN) mutants, and the mutants were identified by their ability to carry out transcription in the absence of yeast chromatin remodeling complex SWI/SNF. SIN mutations are known to affect higher order chromatin structure and the comparative expression profile would help identification of genes which get affected by such altered chromatin landscape.
Keywords: mutant analysis
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| |
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| Overall design |
Comparison of genes whose mRNA levels are affected in histone H4R45H mutant yeast strains compared to wild type yeast strain WY139. This comparison reveals how subtle change in a single amino acid residue in core domain of histone H4 affects gene expression profile in yeast.
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| |
|
| Contributor(s) |
Nag R, Smerdon MJ |
| Citation(s) |
18508805 |
| Submission date |
Apr 28, 2008 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Michael J. Smerdon |
| E-mail(s) |
smerdon@wsu.edu
|
| Phone |
509-335-6853
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
Box 644660
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164-4660 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA106689 |
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