GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM282122 Query DataSets for GSM282122
Status Public on Nov 24, 2008
Title WTGR(-)::2
Sample type RNA
Source name WT Glucocorticoid receptor alpha treated for 0 hr, replicate 2
Organism Homo sapiens
Characteristics Human osteosarcoma, female
Extracted molecule total RNA
Extraction protocol The RNeasy RNA extraction kit, with on column DNAse digestion (Qiagen) was used, according to manufacturer's protocol.
Label Cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description Cells were grown in DMEM-F12 medium, supplemented with 10% Fetal Bovine Serum. 48 hour before treatments, cells were placed in DMEM-F12 medium supplemented with 10% charcoal-dextran-treated FBS. Cells were not treated or were treated with 100 nM dexamethasone for 6 hr.
Data processing Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The Agilent Feature Extraction Software performed error modeling, adjusting for additive and multiplicative noise. The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
Submission date Apr 17, 2008
Last update date Jan 05, 2012
Contact name NIEHS Microarray Core
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
Platform ID GPL4133
Series (1)
GSE11205 Glycogen Synthase Kinase-3-beta Regulates Glucocorticoid Signaling by Phosphorylating the Glucocorticoid Receptor

Data table header descriptions
ID_REF Agilent Feature Number
VALUE Log2 intensity exported from Rosetta Resolver

Data table
44919 11.63359
8563 5.27768
28336 7.42167
5006 15.22712
28801 14.14499
29429 16.56041
38756 8.53014
31922 13.31891
3509 2.91721
27587 12.52884
18581 8.71279
10122 10.36147
14308 5.3476
35097 2.68158
22537 5.32454
1387 10.1135
41336 9.13381
33919 2.49282
24114 14.40071
9363 9.40554

Total number of rows: 41000

Table truncated, full table size 560 Kbytes.

Supplementary file Size Download File type/resource
GSM282122.txt.gz 8.9 Mb (ftp)(http) TXT
GSM282122_US22502625_251485022324_S01_H.tif.gz 62.1 Mb (ftp)(http) TIFF
GSM282122_US22502625_251485022324_S01_L.tif.gz 47.1 Mb (ftp)(http) TIFF
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap