|
| Status |
Public on Jun 05, 2008 |
| Title |
ON_KIDNEY_7 |
| Sample type |
RNA |
| |
|
| Source name |
Renal Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
Age: Unknown
Gender: M
Renal Tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's protocol
|
| Label |
SAPE
|
| Label protocol |
10 ug of Qiagen-purified total RNA was labeled with 6ul of 1mg/ml R-Phycoerythrin Streptavidin (SAPE)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
Clear cell, chromophobe, papillary (types 1 and 2), oncocytoma, and Wilms tumor were compared to normal kidney tissue.
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - The version 6 entrez gene ID CDF was used. Data were then rma normalized using R and the bioconductor suite of analysis tools (eset <- rma(ab))
|
| |
|
| Submission date |
Apr 02, 2008 |
| Last update date |
Jun 05, 2008 |
| Contact name |
Eric Kort |
| Organization name |
Van Andel Research Institute
|
| Street address |
333 Bostwick NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL6671 |
| Series (1) |
| GSE11024 |
Microarray analaysis of adult and childhood renal tumors. |
|
