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Sample GSM2719951 Query DataSets for GSM2719951
Status Public on Jul 28, 2017
Title HGSC_JB5223
Sample type RNA
Source name primary ovarian tumor tissue
Organism Homo sapiens
Characteristics stic: No
age: 66
path.stage: IIIC
surgical.outcome: Optimal
diagnosis.of.record: Ovarian Ca Ovarian Ca
platinumfreeinterval.mos..: 15.7
platinumstatus: Sensitive
recurrence: Y
pfs.mos..: 20.3
os.mos..: 20.3
vitalstatus: Alive
race: WHITE
tcga_subtype_dif: -0.206
tcga_subtype_imr: 0.374
tcga_subtype_mes: -0.47
tcga_subtype_pro: 0.363
tissue: primary tumor tissue from high grade serous ovarian tumors
Extracted molecule total RNA
Extraction protocol DNA and RNA were co-isolated from frozen tissue tumor blocks, and RNA was isolated from normal tissues utilizing Ambion’s ToTALLY RNA™ RNA Isolation Kit (Part Number AM1910, ©Ambion, Inc.). DNA concentration was confirmed using a PicoGreen protocol. RNA quality was confirmed by utilizing an Agilent Bioanalyzer and assigning an RNA Integrity Number (RIN) to each sample.
Label NA
Label protocol Not applicable
Hybridization protocol Total RNA (100 ng) was obtained from 95 of 96 samples, and hybridized to the nCounter Human miRNA sample probes (Seattle, WA).
Scan protocol Each sample was scanned for 600 FOV on the nCounter Digital Analyzer
Description JB5223
Data processing The nanoStringNorm procedure as implemented in the nanoStringNorm Bioconductor package was used to pre-process the miRNA data, followed by differential expression analysis with Empirical Bayes methods via the limma Bioconductor package.
Submission date Jul 27, 2017
Last update date Jul 28, 2017
Contact name leslie cope
Organization name leslie cope
Street address 550 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21209
Country USA
Platform ID GPL17904
Series (2)
GSE101976 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma [miRNA expression]
GSE102094 Molecular analysis of high-grade serous ovarian carcinoma with and without associated serous tubal intra-epithelial carcinoma

Data table header descriptions
VALUE normalized counts

Data table
hsa-let-7a-5p 13.33460836
hsa-let-7b-5p 12.43918385
hsa-let-7c 9.254699952
hsa-let-7d-5p 10.42600935
hsa-let-7e-5p 8.988253249
hsa-let-7f-5p 8.856657481
hsa-let-7g-5p 11.86756252
hsa-let-7i-5p 10.92572199
hsa-miR-1 5.817951717
hsa-miR-100-5p 8.764048854
hsa-miR-101-3p 5.607984343
hsa-miR-103a-3p 4.240298259
hsa-miR-105-5p 5.546809999
hsa-miR-106a-5p+hsa-miR-17-5p 10.03912324
hsa-miR-106b-5p 9.763157143
hsa-miR-107 7.780704485
hsa-miR-10a-5p 10.07966611
hsa-miR-10b-5p 8.193182429
hsa-miR-1178 5.607984343
hsa-miR-1179 4.912700283

Total number of rows: 822

Table truncated, full table size 20 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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