|
| Status |
Public on Feb 22, 2019 |
| Title |
MDA-MB231-1833 cells with GLG1 KO by CRISPR, seeded on E-Selectin |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MDA-MB231-1833 cells with GLG1 KO by CRISPR, seeded on E-Selectin
|
| Organism |
Homo sapiens |
| Characteristics |
parental cell line: MDA-MB-231
subline: 1833
crispr ko: GLG1
treatment: E-Selectin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using RNAeasy mini-prep kit from Qiagen
|
| Label |
Cy5
|
| Label protocol |
The testing RNA samples and universal mouse reference RNA (Agilent) were labeled with CTP-cy5 and CTP-cy3, respectively, Using Agilent Quick Amp Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
Universal human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell lines: Mix of 10 human cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using RNAeasy mini-prep kit from Qiagen
|
| Label |
Cy3
|
| Label protocol |
The testing RNA samples and universal mouse reference RNA (Agilent) were labeled with CTP-cy5 and CTP-cy3, respectively, Using Agilent Quick Amp Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
Each cy5 labeled testing sample was mixed with equal amount cy3-labeled reference sample and fragmented and blocked (Agilent Gene Expression Hybridization Kit), and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version 11.0).
|
| Description |
See Agilent Cat#740000 for product details
Cells transduced with CRISPR vectors targeting Glg1 or Control were seeded on E-selectin or IgG (10 ug/mL) coated plates for 12 h
|
| Data processing |
Agilent Feature Extraction Software (v 11.0) was used for background subtraction and linear Lowess normalization.
|
| |
|
| Submission date |
Mar 17, 2017 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Yong Wei |
| E-mail(s) |
yongwei@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Kang
|
| Street address |
LTL227 Wathington Road
|
| City |
Plainsboro |
| State/province |
NJ |
| ZIP/Postal code |
08536 |
| Country |
USA |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE96754 |
Endosteal Niche E-selectin Induces Mesenchymal-Epithelial Transition and Wnt Activation in Cancer Cells to Promote Bone Metastasis |
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