|
| Status |
Public on Mar 16, 2020 |
| Title |
Melanoma cell line subclone 2 MCM1D |
| Sample type |
genomic |
| |
|
| Source name |
Melanoma cell line MCM1D
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: melanoma
origin: Melanoma cell line MCM1D derived from MCM1
cell malignancy: tumor forming and metastasizing to lymph nodes after injection into SCID mice
gender: male
|
| Growth protocol |
Melanoma cells were cultured in MIM medium supplemented with 2 % FCS containing: 80 % MCDB153, 20 % Leibovitz`s l15, 5 µg/ml Insuline, bovine, 0.5 ng/ml Epidermal Growth Factor (EGF), 1.68 mM CaCl2, 100 IU/ml Penicillin, 100 µg/ml Streptomycin and 2 µg/ml Ciprofloxacin at 37°C at 5% CO2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated by using the All Prep DNA Kit (Qiagen, Hilden, Germany) according to the manufacturer`s protocol.
|
| Label |
biotin
|
| Label protocol |
According to the manufacturer`s protocol (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled, and hybridized to each array as per manufacturer`s instructions (Affymetrix).
|
| Scan protocol |
The arrays were scanned using the appropriate Gene Chip Scanner.
|
| Data processing |
CEL files were processed via the genotyping console 4.2.0 using default parameters. CHP files were generated via the genotyping console 4.2.0 using default parameters. Normalization was performed using the birdseed v2 algorithm.
primary data description: Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
Mar 16, 2017 |
| Last update date |
Mar 16, 2020 |
| Contact name |
David Schörghofer |
| E-mail(s) |
david.schoerghofer@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Department for Pathobiochemistry and Genetics
|
| Street address |
Währingerstraße 10
|
| City |
Wien |
| ZIP/Postal code |
1200 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE96708 |
Affymetrix SNP6.0 array data for isogenic melanoma cell lines |
|
