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Series GSE96708 Query DataSets for GSE96708
Status Public on Mar 16, 2020
Title Affymetrix SNP6.0 array data for isogenic melanoma cell lines
Organism Homo sapiens
Experiment type SNP genotyping by SNP array
Summary Here we report on the DNA profile of the isogenic melanoma cell lines MCM1, MCM1G, MCM1D and MCM1DLN. MCM1 cells are melanoma cells generated from a nodular melanoma stage T2a, N0 of a 61 year old male patient (see GSE35991). Two subclones were derived from MCM1 termed MCM1G and MCM1D. When transplanted into SCID mice, MCM1, MCM1G and MCM1D formed tumors at the injection site. However, only MCM1D metastasized to lymph nodes. Subsequently, MCM1D cells were isolated from the lymph nodes of SCID mice and termed MCM1DLN. Upon reimplantation into SCID mice, MCM1DLN cells showed a stable metastatic phenotype. Interestingly, MCM1D and MCM1DLN tumors showed reduced NLGN4X expression on the mRNA and protein level, suggesting a role in melanoma progression.
Overall design Affymetrix SNP arrays were performed according to the manufacturer`s instructions. DNA was extracted from the melanoma cell line MCM1 and its derived subclones MCM1G, MCM1D and MCM1DLN.
Contributor(s) Schörghofer D, Mikula M
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Submission date Mar 16, 2017
Last update date Mar 17, 2020
Contact name David Schörghofer
Organization name Medical University of Vienna
Department Department for Pathobiochemistry and Genetics
Street address Währingerstraße 10
City Wien
ZIP/Postal code 1200
Country Austria
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (4)
GSM2538783 Melanoma cell line MCM1
GSM2538784 Melanoma cell line subclone 1 MCM1G
GSM2538785 Melanoma cell line subclone 2 MCM1D
BioProject PRJNA379435

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE96708_RAW.tar 173.1 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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