|
| Status |
Public on Apr 29, 2008 |
| Title |
Second trimester placenta Grp2H17812 |
| Sample type |
RNA |
| |
|
| Source name |
placenta
|
| Organism |
Homo sapiens |
| Characteristics |
Collection of human placentae was approved by the Institutional Review Board of the University of Washington. Subjects were excluded if they had any chronic disease, were on chronic medication, were smokers, abused alcohol or drugs. Term human placenta samples were obtained from scheduled uncomplicated C-sections performed at the University of Washington Medical Center, Seattle, WA. The 1st (45-59 days) and 2nd (109-115 days) trimester placentae, from uncomplicated elective termination, were provided by Birth Defects Laboratory of the University of Washington. After collection, the tissues were kept on ice until the placental villus could be dissected and snap-frozen in liquid nitrogen and stored at -80C.
|
| Biomaterial provider |
University of Washington Medical Center
|
| Treatment protocol |
Term human placenta samples were obtained from scheduled uncomplicated C-sections performed at the University of Washington Medical Center, Seattle, WA. The 1st (45-59 days) and 2nd (109-115 days) trimester placentae, from uncomplicated elective termination, were provided by Birth Defects Laboratory of the University of Washington.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the placental tissue using the RNAesy kit (Quigen) according to the manufacturer's protocol.!Sample_label = biotin
|
| Label |
biotin
|
| Label protocol |
Total RNA (2 ug) was labeled and hybridized to the Affymetrix Human Genome U133 Plus 2.0 arrays and hybridized according to manufacurer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
Term human placenta samples were obtained from scheduled uncomplicated C-sections performed at the University of Washington Medical Center, Seattle, WA. The 1st (45-59 days) and 2nd (109-115 days) trimester placentae, from uncomplicated elective termination, were provided by Birth Defects Laboratory of the University of Washington.
|
| Data processing |
GeneTraffic RMA normalization log2
|
| |
|
| Submission date |
Dec 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9984 |
Profiling Gene Expression in Human Placentae of Different Gestational Ages: an OPRU Network and UW SCOR Study |
|
| Relations |
| Reanalyzed by |
GSE119087 |
