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Sample GSM2433489 Query DataSets for GSM2433489
Status Public on Dec 05, 2019
Title H1299-con sh
Sample type RNA
Source name non small lung cancer cell
Organism Homo sapiens
Characteristics cell line: H1299
cell type: non small lung cancer cell
tissue: lung
genotype/variation: control
Treatment protocol CNOT2 gene was knocked down for 48 hr and melatonin 1mM was treated for 24 hr.
Growth protocol A549 and H1299 were grown in RPMI with 10% FBS and antibiotics. XO1 and XO2 were grown in DMEM-F12 with B27 and antibiotics supplemented with growth factors (EGF and bFGF).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions.
Label Cy3
Label protocol Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
Hybridization protocol After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Human Oligo Microarray (60K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).
Scan protocol The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).
Description 3. H1299-con sh
Data processing All data normalization and further analysis were performed using GeneSpringGX 7.3 (Agilent Technology, USA).
Submission date Dec 20, 2016
Last update date Dec 05, 2019
Contact name Hyemin Lee
Organization name Kyunghee Univ
Street address Kyunghee-dae-ro 26
City Seoul
ZIP/Postal code 131701
Country South Korea
Platform ID GPL17077
Series (1)
GSE92612 CNOTshRNA or Melatonin treated samples

Data table header descriptions
VALUE Log2 normalized signal intensity (Global normalization)

Data table
A_19_P00315452 9820.663
A_19_P00315459 349.2484
A_19_P00315482 18.18621
A_19_P00315492 20.45976
A_19_P00315493 877.1326
A_19_P00315502 4.14069
A_19_P00315506 11.65507
A_19_P00315518 4.748864
A_19_P00315519 6.120944
A_19_P00315524 23.5959
A_19_P00315528 135.5387
A_19_P00315529 131.9681
A_19_P00315538 4.854194
A_19_P00315541 5.211428
A_19_P00315543 90.09159
A_19_P00315550 230.1989
A_19_P00315551 281.8531
A_19_P00315554 13.27068
A_19_P00315581 6238.768
A_19_P00315583 820.7445

Total number of rows: 50599

Table truncated, full table size 1108 Kbytes.

Supplementary file Size Download File type/resource
GSM2433489_3._H1299-con_sh.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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