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Sample GSM2386165 Query DataSets for GSM2386165
Status Public on Aug 01, 2017
Title WT Treg (GFP+ from A384T:hCD2/WT:GFP mice)_1
Sample type RNA
Source name hCD2(-)GFP(+)CD4(+) cells sorted from FoxP3A384T:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(-)GFP(+)CD4(+) cells
sorted from: FoxP3A384T:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.36760044
AFFX-BioB-M_at -0.39350986
AFFX-BioB-3_at -0.38601017
AFFX-BioC-5_at -0.37847233
AFFX-BioC-3_at -0.27845
AFFX-BioDn-5_at -0.37254047
AFFX-BioDn-3_at -0.51580715
AFFX-CreX-5_at -0.3391657
AFFX-CreX-3_at -0.35239506
AFFX-DapX-5_at -1.1163816
AFFX-DapX-M_at -0.8023834
AFFX-DapX-3_at -0.9870558
AFFX-LysX-5_at -0.6318464
AFFX-LysX-M_at -0.96222305
AFFX-LysX-3_at -0.98814964
AFFX-PheX-5_at -1.1494637
AFFX-PheX-M_at -1.172596
AFFX-PheX-3_at -1.2533388
AFFX-ThrX-5_at -0.8297787
AFFX-ThrX-M_at -1.1632442

Total number of rows: 45101

Table truncated, full table size 1010 Kbytes.

Supplementary file Size Download File type/resource
GSM2386165_RSM00118.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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